Journal: bioRxiv
Article Title: Pharmacological interrogation of crosstalk between TLR signaling and stress granules reveals a compound with antiviral effect
doi: 10.1101/2025.04.11.648452
Figure Lengend Snippet: A ) Western blot validation of gene knockouts for the three G3BP1 -/- A549 cells clones. B ) Immunofluorescence confocal imaging of mock treated WT and G3BP1 -/- A549 cells to visualize G3BP1 expression and SGs. C ) Immunofluorescence confocal imaging of PPM18 treated WT and G3BP1 -/- A549 cells to visualize G3BP1 expression and SGs. D ) Quantification of SG + WT and G3BP1 -/- A549 cells upon PPM18 treatment. One-way ANOVA was used to determine the statistical significance. **** p -value<=0.0001. G3BP1 was immunostained with AlexaFluor488 secondary antibody. DAPI was used to visualize nuclei. DDX3X used as the alternate SG marker.
Article Snippet: Cells were stained with mouse anti-G3BP1 (1:1000; 27299-I-AP; Proteintech), goat anti-Influenza A Virus polyclonal antibody (abcam, ab20841) or rabbit anti-DDX3X (1:1000; A300-474A; Bethyl Laboratories) antibody overnight at 4°C.
Techniques: Western Blot, Clone Assay, Immunofluorescence, Imaging, Expressing, Marker